Cells Proliferation Control Medium
72hr change-free medium culture of human ES/iPS cells in Xyltech BOF-01
♠ BOF-01 is a novel basal culture medium which can suppress proliferation of human ES/iPS cell on feeder cell layers.
♠ BOF-01 can be used by completely replacing the basal culture medium (e.g. DMEM/F12) of the human ES/iPS cells.
♠ Human ES/iPS cells can be maintained with BOF-01 for about 3 days (up to 72 hours) with out changing medium under normal culture conditions (37ºC, 5% CO2).
♠ After suppression of cell proliferation with BOF-01, cell growth can be resumed by changing normal growth medium.
♠ This product does not contain glucose.
Overview of Weekend-free Protocol
Morphology of Undifferentiated Cell Colony and Effect of Proliferation Control
Undifferentiation markers expression in Human iPS cells by BOF-01
Differentiation of Three Germ Layers (Teratoma Assay)
Colony Morphology changes during Proliferation Control Culture
